bitorquis mycelia is capable of stimulating primordial instead of non-living bacterial suspensions. Primordium stimulation assay was registered by photographs, and micrographs of vertical cut of primordium were registered by scanning electron microscope. bitorquis as a primordium stimulation model. putida and non-living bacterial suspensions, after chemical or physical treatments, was tested using A. In vitro primordium stimulation of living P. Four biochemical assays were used to identify P. bisporus cultivation and among those 12 % are Pseudomonas putida. corresponds to 75–85 % of bacterial population of the casing layers in A. bisporus cultivation were collected and quantified. communities of different casing layers of A. bisporus cultivation, isolate, identify and characterize the bacteria responsible for the stimulation of primordium and their action mechanism using Agaricus bitorquis as a primordium stimulation model. Thus, the objective of this study was to characterize bacterial microbiota of casing layers from A. bisporus is not able to produce primordia in sterile casing layers or Petri dishes. ![]() ![]() It is suggested that a minimal community of these microorganisms would be necessary to stimulate fructification. Casing layer is one step of Agaricus bisporus cultivation where there is a competitive environment with a high number of microorganisms and diversity interacting with mycelia.
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